brown beverage

New publication investigating mode of action for high-dose effects of 4-methylimidazole

Patrick McMullen Blog

by Patrick McMullen We are pleased to announce our most recent manuscript, A systematic approach to evaluate plausible modes of actions for mouse lung tumors in mice exposed to 4-methylimidazole, published in Regulatory Toxicology and Pharmacology. 4-methylimidazole is a ubiquitous compound present in foods that undergo thermal browning (i.e., Maillard reaction) processes. The work described here—a combined effort of investigators from ScitoVation and ToxStrategies supported by the American Beverage Association—aimed …

A graph showing value of KMD

The Importance of using Kinetically Derived Maximum Dose (KMD)

ScitoVation Blog

KMD allows for design of in-life experiments that are not confounded by non-linear effects, including saturation of absorption processes. This results in a data package that is more easily interpreted because of its increased relevance to human exposures.    by Jeff Fisher For those who design repeated dose, subchronic and chronic animal toxicity studies, this concept may be an additional responsibility for you.  Gone are …

Damaged DNA

Discovery of a new mechanism for genetic change: Exitron splicing implications for carcinogenesis

ScitoVation Blog

by Michael Black   Carcinogenesis, or the process of transformation of cells to a cancerous state, often is associated with aberrations in messenger RNA splicing. Removal of introns in pre-mRNA is an essential step in normal cell processing of mRNA prior to transcription of a peptide from a final mRNA transcript. Alternative splicing of pre-mRNA gives cells the ability to regulate multiple final mRNA products from a single gene, adding diversity to the …

3D heart

New Approach Methodologies for Environmental Cardiotoxicity Testing

ScitoVation Blog

by Marjory Moreau Recently I attended  a webinar on “Cardiac toxicity Evaluation with a human tissue-engineered model” by Kareen Coulombe, professor at Brown University. I am still blown away every time I hear Dr. Coulombe speak on this subject. In addition to being an excellent speaker, the work she and her team are doing is incredible. I recently wrote a blog on the brain and all its complexity because the brain is not an organ I had worked …

Descriptive words blog

The Need for Increased Maturity in the Risk Assessment Ecosystem

ScitoVation Blog

by Dr. Jean Orelien Last year, the US EPA declared they would phase away from animal testing by 2035. Reaching this milestone will require the maturity of the risk assessment ecosystem. By maturity, I mean new roles need to be assumed along with improved interactions and (stronger) bonds within and between group as well as new entities. In this post, I share a …

representing 3D liver models

Exploring In Vitro 3D Liver Models: Complexity Demands Different Needs

ScitoVation Blog

by Aarati Ranade Every time we perform an experiment with hepatocytes, I am curious to see how the culture looks over time after plating the cells. I carefully place the cell culture plate under the microscope. I carefully observe through the microscope eyepiece. The transition from round cells to formation of flat, hexagonal cells with one or two prominent nuclei with cell borders nicely touching each other to form a carpet-like monolayer …

Alternatives to Animal Testing

Toward More Efficient Safety Testing Through Development of an Internal Threshold of Toxicological Concern

ScitoVation Blog

by Alina Y. Efremenko One of my memorable moments when I was a teenager was my family’s trip to the Grand Canyon. That was my first experience riding a horse. I begged my grandfather to come with me, and reluctantly he agreed. I asked him why he disliked the idea so much and he said, “No one asked the horses.” …

High Content Imaging Delivers the Best Results for Microscopy

ScitoVation Blog

Microscopy is a technique for capturing images of tiny objects, and it is an invaluable tool for investigating cells and tissues and their molecular constituents.  Microscopy is particularly well-suited for assessment of cell, tissue, or organelle morphology; determination of macromolecule expression level, subcellular localization, translocation, or co-localization; and the measurement of cellular heterogeneity or the identification and study of cellular …

Physiologically Based Pharmacokinetic (PBPK) Modeling of Central Nervous System

ScitoVation Blog

by Marjory Moreau, Ph.D. I have worked in PBPK modeling for many years and until a few months ago, I never had to deal with the brain compartment. Well, I had a brain compartment in several of my models, but I never really thought about the blood brain barrier and all the transporters involved in protecting or bringing compounds into the brain until recently. I …