In Vitro Inhalation Point-of-Contact Toxicity Testing with Vapors

Thanks to all who joined us on Thursday, April 29th, at 11am for a webinar highlighting alternatives to in vivo animal testing that instead leverage PBPK modeling, presented by Dr. Scott Slattery, a cell biologist at ScitoVation.

Abstract

In vitro chemical risk assessment using human cells is emerging as an alternative to in vivo animal testing with reduced costs, fewer animal welfare concerns, and the possibility of greater human health relevance.  In vitro inhalation toxicity testing of volatile compounds poses particular challenges.  We will discuss our efforts to expose 3D human airway epithelial cultures to vapors at the air–liquid interface and to assess adverse responses.  Using methyl iodide (MeI) and 1,3-dichloropropene (DCP) as test compounds, we have examined viability, cytotoxicity, and epithelial integrity responses after acute and repeated exposures.  Using multiple cell culture models that represent different regions of the human airway, we have determined tissue-specific in vitro points of departure for DCP.  We have translated these in vitro point-of-departure values to equivalent inhaled concentrations by reverse dosimetry using a PBPK model.  These calculated equivalent inhaled concentrations may enable prediction of in vivo points of departure.   

About Dr. Scott Slattery

Dr. Scott Slattery is a cell biologist at ScitoVation.  He received his PhD from Baylor College of Medicine, where he studied mitotic regulators.  As a postdoc at the University of North Carolina – Chapel Hill, he worked on the development of genetically expressed, FRET-based sensors for studying signaling process with high spatiotemporal resolution.  At ScitoVation, Scott is focused on assay development, including in vitro inhalation toxicology.

About ScitoVation

ScitoVation helps clients assess chemical compound safety using innovative science, next-generation technology, and professional expertise. ScitoVation is known for partnership, flexibility, and proven success in its work to develop safer and more effective pharmaceuticals, food ingredients, agricultural chemicals, commodity chemicals, and consumer products.

As a spin-off of former The CIIT and The Hamner Institutes for Chemical & Drug Safety Sciences, ScitoVation is an industry leader of New Approach Methods (NAMS) for chemical/drug discovery & development in the rapidly evolving global regulatory landscape.