LRI Tools and Methods: In Vitro to In Vivo Extrapolation (IVIVE)

How do we integrate bioactivities generated from our in vitro assays into our safety decision making process?

The development of in silico and in vitro approaches is accelerating the movement away from animal studies in chemical safety testing. These technologies have the potential to increase confidence and throughput of chemical safety decision making. A critical component in redirecting toxicity testing towards high-throughput methodologies is the development of techniques for quantitatively comparing bioactivity levels seen in new approach methodologies (NAMs) and estimated human exposures. In vitro-to-in vivo extrapolation (IVIVE) provides a quantitative framework for converting bioactivity values derived from in vitro testing to an equivalent external exposure. This conversion allows direct comparison between chemical hazard levels and anticipated exposure scenarios, resulting in more relevant safety decision making. PLETHEM, the pharmacokinetics platform developed through ACC LRI, provides an easy-to-use interface and workflows to perform IVIVE.

Resources

PLETHEM User Guides and Additional Information